| Product Name |
Recombinant Human Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Protein |
| Product Overview |
This recombinant human Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) protein includes amino acids 1-164aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P38936 |
| Recommended Name |
Cyclin-dependent kinase inhibitor 1 |
| Gene Name |
CDKN1A |
| Synonyms |
Cyclin-dependent kinase inhibitor 1A, P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1. |
| Species |
Human |
| Predicted Molecular Mass |
20.2 kDa |
| Expression System |
E.coli |
| Expression Range |
1-164aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Cycle |
| Target Function |
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Protein Family |
CDI family |
| Tissue Specificity |
Expressed in all adult tissues, with 5-fold lower levels observed in the brain. |
