| Description |
Recombinant Human CD8 alphaβ Heterodimer Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
P01732-1(CD8 alpha)&P10966-1(CD8 beta) |
| Target Symbol |
CD8 alphaβ |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His-Flag |
| Expression Range |
Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail |
| Mol. Weight |
The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 26-28 kDa (CD8 alpha) and 28-32 kDa (CD8 beta) based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human CD8A&CD8B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
CD8 alphaβ (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR. |
