| Product Name |
Recombinant Human Eukaryotic Sialic Acid Binding Ig Like Lectin 2 (CD22) Protein |
| Product Overview |
This recombinant human Eukaryotic Sialic Acid Binding Ig Like Lectin 2 (CD22) protein includes amino acids 20-400aa of the target gene is expressed in 293F cell.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P20273 |
| Recommended Name |
B-cell receptor CD22 |
| Gene Name |
CD22 |
| Synonyms |
SIGLEC-2;SIGLEC2 |
| Species |
Human |
| Predicted Molecular Mass |
120 kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
20-400aa |
| Tag |
C-Fc |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer, Immunology |
| Target Function |
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. |
| Subcellular Location |
Cell membrane; Single-pass type I membrane protein. |
| Protein Family |
Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family |
| Tissue Specificity |
B-lymphocytes. |
