| Product Name |
Recombinant Human Beta Actin (ACTB) Protein |
| Product Overview |
This recombinant human Beta Actin (ACTB) protein includes amino acids 313-547aa of the target gene is expressed in E.coli.The protein is supplied in liquid or lyophilized powder form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P60709 |
| Recommended Name |
Actin, cytoplasmic 1 |
| Gene Name |
ACTB |
| Synonyms |
G24; MRPL56; Mitochondrial Ribosomal Protein L56; Serine beta-lactamase-like protein LACTB, mitochon |
| Species |
Human |
| Predicted Molecular Mass |
30.3 kDa |
| Expression System |
E.coli |
| Expression Range |
313-547aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Liquid or Lyophilized powder |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. |
| Subcellular Location |
Cytoplasm, cytoskeleton. Nucleus. |
| Protein Family |
Actin family |
| Associated Diseases |
Dystonia, juvenile-onset (DJO); Baraitser-Winter syndrome 1 (BRWS1) |
