| Product Name |
Recombinant Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) Protein |
| Product Overview |
This recombinant human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) protein includes amino acids 798-896 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
P20648 |
| Recommended Name |
Potassium-transporting ATPase alpha chain 1 |
| Gene Name |
ATP4A |
| Synonyms |
Gastric H,K-ATPase Alpha Subunit; H(+)-K(+)-ATPase Alpha Subunit; Proton Pump; Potassium-transporting ATPase alpha chain 1 |
| Species |
Human |
| Predicted Molecular Mass |
17kDa |
| Expression System |
E.coli |
| Expression Range |
798-896 |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Others |
| Target Function |
The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state. |
| Subcellular Location |
Apical cell membrane; Multi-pass membrane protein. |
| Protein Family |
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily |
| Tissue Specificity |
Expressed in gastric parietal cells (at protein level). |
