| Product Name |
Recombinant Human ANG Protein |
| Product Overview |
This recombinant human ANG protein includes amino acids 25-147aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P03950 |
| Recommended Name |
Angiogenin |
| Gene Name |
ANG |
| Synonyms |
Angiogenin, ribonuclease, RNase A family, 5, ALS9, HEL168, MGC22466, MGC71966, RNASE4, RNASE5 |
| Species |
Human |
| Predicted Molecular Mass |
16.4 kDa |
| Expression System |
E.coli |
| Expression Range |
25-147aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Target Function |
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. |
| Subcellular Location |
Cytoplasmic vesicle, secretory vesicle lumen. Secreted. Nucleus. Nucleus, nucleolus. |
| Protein Family |
Pancreatic ribonuclease family |
| Associated Diseases |
Amyotrophic lateral sclerosis 9 (ALS9) |
| Tissue Specificity |
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. |
