| Product Name |
Recombinant Human ALKBH2 Protein |
| Product Overview |
This recombinant human ALKBH2 protein includes amino acids 1-261aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q6NS38 |
| Recommended Name |
DNA oxidative demethylase ALKBH2 |
| Gene Name |
ALKBH2 |
| Synonyms |
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, ABH2, Oxy DC1. |
| Species |
Human |
| Predicted Molecular Mass |
31.4 kDa |
| Expression System |
E.coli |
| Expression Range |
1-261aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. |
| Subcellular Location |
Nucleus. Note=Detected in replication foci during S-phase. |
| Protein Family |
AlkB family |
| Tissue Specificity |
Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. |
