| Product Name |
Recombinant Human Dihydrodiol Dehydrogenase 2 (DDH2/AKR1C2) Protein |
| Product Overview |
This recombinant human Dihydrodiol Dehydrogenase 2 (DDH2/AKR1C2) protein includes amino acids 1-323aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P52895 |
| Recommended Name |
Aldo-keto reductase family 1 member C2 |
| Gene Name |
AKR1C2 |
| Synonyms |
Aldo-Keto Reductase Family 1 Member C2; 3-Alpha-HSD3; Chlordecone Reductase Homolog HAKRD; Dihydrodi |
| Species |
Human |
| Predicted Molecular Mass |
42 kDa |
| Expression System |
E.coli |
| Expression Range |
1-323aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism |
| Target Function |
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids. |
| Subcellular Location |
Cytoplasm, cytosol. |
| Protein Family |
Aldo/keto reductase family |
| Associated Diseases |
46,XY sex reversal 8 (SRXY8) |
| Tissue Specificity |
Expressed in fetal testes. Expressed in fetal and adult adrenal glands. |
