| Product Name |
Recombinant Human Aldose Reductase Like 1 (ARL1/AKR1B10) Protein |
| Product Overview |
This recombinant human Aldose Reductase Like 1 (ARL1/AKR1B10) protein includes amino acids 1-316 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
O60218 |
| Recommended Name |
Aldo-keto reductase family 1 member B10 |
| Gene Name |
AKR1B10 |
| Synonyms |
AKR1B10; AKR1B12; HIS; HSI; ALDRLn; Aldo-Keto Reductase Family 1,Member B10; Aldose Reductase-Related Protein; Small Intestine Reductase |
| Species |
Human |
| Predicted Molecular Mass |
50kDa |
| Expression System |
E.coli |
| Expression Range |
1-316 |
| Tag |
N-6His+sumo |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose. |
| Subcellular Location |
Lysosome. Secreted. Note=Secreted through a lysosome-mediated non-classical pathway. |
| Protein Family |
Aldo/keto reductase family |
| Tissue Specificity |
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. |
