| Product Name |
Recombinant Human Acyl Coenzyme A Dehydrogenase, C4-To-C12 Straight Chain (ACADM) Protein |
| Product Overview |
This recombinant human Acyl Coenzyme A Dehydrogenase, C4-To-C12 Straight Chain (ACADM) protein includes amino acids 191-421aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P11310 |
| Recommended Name |
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial |
| Gene Name |
ACADM |
| Synonyms |
Medium-chain specific acyl-CoA dehydrogenase; ACAD1; CAD; MCADH. |
| Species |
Human |
| Predicted Molecular Mass |
29 kDa |
| Expression System |
E.coli |
| Expression Range |
191-421aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains. |
| Subcellular Location |
Mitochondrion matrix. |
| Protein Family |
Acyl-CoA dehydrogenase family |
| Associated Diseases |
Acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) |
