| Product Name |
Recombinant Human Permeability Glycoprotein (Pgp/ABCB1) Protein |
| Product Overview |
This recombinant human Permeability Glycoprotein (Pgp/ABCB1) protein includes amino acids 1023-1280aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P08183 |
| Recommended Name |
ATP-dependent translocase ABCB1 |
| Gene Name |
ABCB1 |
| Synonyms |
CD243; ABCB1; ABC20; CLCS; GP170; MDR1 |
| Species |
Human |
| Predicted Molecular Mass |
32 kDa |
| Expression System |
E.coli |
| Expression Range |
1023-1280aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Transport |
| Target Function |
Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. |
| Subcellular Location |
Cell membrane; Multi-pass membrane protein. Apical cell membrane. |
| Protein Family |
ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily |
| Associated Diseases |
Inflammatory bowel disease 13 (IBD13) |
| Tissue Specificity |
Expressed in liver, kidney, small intestine and brain. |
