| Product Name |
Recombinant Human α-Synuclein (SNCA) Protein |
| Product Overview |
This recombinant human α-Synuclein (SNCA) protein includes amino acids 61-140aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in 20 mM Tris-HCl buffer (pH 7.5) containing 0.1 M NaClprior to lyophilization. |
| Target Uniprot Id |
P37840 |
| Recommended Name |
Alpha-synuclein |
| Gene Name |
SNCA |
| Synonyms |
SNCA, NACP, PARK1, alpha-Synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Alpha synuclein, Alpha-synuclein isoform NACP140, alphaSYN, MGC105443, MGC110988, MGC127560, MGC64356 |
| Species |
Human |
| Predicted Molecular Mass |
8.4 kDa (81 aa) |
| Expression System |
E.coli |
| Expression Range |
61-140aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
20 mM Tris-HCl buffer (pH 7.5) containing 0.1 M NaCl |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Neuroscience |
| Target Function |
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
| Subcellular Location |
Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Secreted. |
| Protein Family |
Synuclein family |
| Associated Diseases |
Parkinson disease 1, autosomal dominant (PARK1); Parkinson disease 4, autosomal dominant (PARK4); Dementia Lewy body (DLB) |
| Tissue Specificity |
Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain. |
