Pfu DNA Polymerase

Description

Pfu DNA polymerase is purified from E. coli carrying a vector with the gene encoding Pyrococcus furiosus DNA polymerase. It has an intrinsic 3′ to 5′ exonuclease activity. It is a highly accurate DNA polymerase than Taq DNA polymerase (about 10-fold).

Applications

• High-fidelity PCR and primer-extension reactions.
• PCR products for cloning and expression.
• PCR cloning and blunt-end amplification.
• Gene synthesis.
• Site-directed mutagenesis.

Key Features

• High fidelity PCR: Proofreading activity with 3′ → 5′ exonuclease.
• High specificity: Better specificity than Taq DNA polymerase with reduced chance of non-specific products.
• Terminal transferase activity: Guaranteed blunt-ended PCR product by blocking terminal transferase activity.