Norovirus (NOV) One-step RT-qPCR Detection (TBS42036)

Description

Norovirus (NOV) is a ubiquitous pathogen that causes gastroenteritis worldwide. The pathogen is an RNA virus belonging to the virus family Caliciviridae. As a highly contagious virus, there are often major outbreaks, particularly in facilities such as child daycares, schools, or hospitals. Symptoms may include diarrhea, nausea, stomach cramps, and vomiting. Severe cases have been reported caused by contaminated fruits, berries, raw vegetables, raw meat, seafood, and bottled water.

Norovirus (NOV) One-step TaqMan Probe RT-PCR Detection Kit combines both reverse transcription and real-time qPCR amplification into a one-step system in a single reaction tube. It makes easier and more accurate to detect NOV contamination from food or vegetable samples. The kit is optimized for the two reactions in a real-time “single step”. This offers the end-users an efficient, easy to use, and reliable alternative to conventional “two-step” sequential RT-qPCR for detection of NOV GI and GII.

NOV One-step TaqMan Probe RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer-probe, internal control, positive control, negative control, and buffer. The NOV target gene GI and GII are labeled with fluorescence Fam, and internal control is labeled with Hex.

Applications

This kit is used for NOV GI and GII detection from food samples.

Key features

• One-step complete qRT-PCR in a single tube.
• Reduce contamination in the operating process.
• Accurate detection and quantification of NOV target gene GI and GII.
• 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.

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