NHI-2, LDHA inhibitor (TBI4555)

Description

Product Details

Formal Name: Methyl 1-Hydroxy-6-phenyl-4-trifluoromethyl-1H-indole-2-carboxylate

Molecular Formula: C₁₇H₁₂F₃NO₃

Formula Weight: 335.28

CAS Number: 1269802-97-2

Purity: >98%

Formulation: powder

Solubility: Soluble in DMSO (up to 25 mg/ml).

Storage: -20°C

Stability: ≥ 1 year.

Applications

LDHA inhibitor

Functions

NHI-2 is a selective inhibitor of Lactate Dehydrogenase A (LDHA) – IC50 in vitro = 14.7 µM (NADH), 10.5 µM (Pyruvate). NHI-2 has also shown cellular growth inhibitory effects against pancreatic cancer cells (PANC-1 cells IC50 = 22.2 µM normoxic and 4.0 µM hypoxic; LPC006 cells IC50 = 17.8 µM normoxic and 1.1 µM hypoxic) with increased efficacy in a cancer cells hypoxic environment. LDHA is a key enzyme involved in the Warburg effect and a cancer cells ability to survive in a hypoxic environment.

Application Procedures

First dissolved in DMSO (up to 25 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20°C for up to 1 month.