NADH/NAD Quantification Colorimetric Assay (TBS2029)

Description

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins.

The Tribio™ NADH/NAD Quantification Colorimetric Assay is based on a dehydrogenase coupled reaction to convert WST-8 to WST-8 formazan, which can be measured at OD 460 nm. The generated signal is proportional to the content of NADH. This assay increases the detection sensitivity by purification of NAD and NADH from the cell lysate. The kit provides the easiest and most accurate approach to measuring NADH from a variety of samples.

Applications

• Cell and biological tissues.

Storage Conditions

Store the Reagent at -20°C protected from light. Shelf life: 6 months.

Standard Curve