| Description |
Recombinant Mouse Integrin alpha 2 beta 1(ITGA2&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr27-Thr1129(ITGA2) acidic tail & Gln21-Asp728(ITGB1) basic tail. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q62469(ITGA2)&P09055-1(ITGB1) |
| Target Symbol |
Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer |
| Synonyms |
ITGA2&ITGB1 ITGA2; ITGB1; |
| Species |
Mouse |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Tyr27-Thr1129(ITGA2) acidic tail & Gln21-Asp728(ITGB1) basic tail |
| Mol. Weight |
The protein has a predicted MW of 126.60 kDa(ITGA2)&82.80 kDa(ITGB1). Due to glycosylation, the protein migrates to 128-138 kDa(ITGA2)&105-115 kDa(ITGB1) based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Integrin alpha 2 beta 1 is a positive regulator of collagenase (MMP-1) and collagen alpha 1(I) gene expression.HOS cells normally lacking alpha 2 beta 1 integrin were forced to express it, and this prevented the down-regulation in the levels of alpha 1 (I) collagen mRNA when cells were grown inside collagen gels. The data indicate that the level of MMP-1 expression is regulated by the collagen receptor alpha 2 beta 1 integrin. |
