Liver Dissociation Kit for Hepatic Cell Profiling

Overview

The The Liver Dissociation Kit provides a powerful enzymatic solution designed to dissociate liver tissue into single-cell suspensions for high-throughput applications. This kit enables researchers to study liver cellular heterogeneity with high viability and efficiency, supporting advanced liver disease research and drug development.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Single-cell analysis of liver tissue is key to understanding diseases like hepatitis, fibrosis, and liver cancer.
  • Traditional dissociation methods often fail to preserve fragile liver cell subtypes or yield complete suspensions.
  • This kit facilitates:
    • Identification of diverse liver cell types, including hepatocytes, Kupffer cells, and stellate cells.
    • Investigation of disease mechanisms, such as inflammation, fibrosis, and tumor progression.
    • Discovery of biomarkers and therapeutic targets for liver-related pathologies.

• Background of Technological Development:

  • Overcomes limitations of cell damage and inefficiency in mechanical or chemical methods.
  • The leverages:
    • Specially selected enzymes targeting liver extracellular matrix.
    • Optimization of enzyme type, concentration, and incubation parameters.
    • A high-yield workflow for producing viable, reproducible single-cell suspensions from liver tissue.

Detection Principle

• Operates on a two-step enzymatic digestion method:
  • Liver tissue is cut into small fragments for uniform exposure.
  • Enzyme reagents are applied under precisely controlled temperature and time conditions.
  • The matrix and cellular junctions are enzymatically degraded.
• Final Result:
  • A high-quality, viable single-cell suspension ready for applications such as scRNA-seq, immunophenotyping, or cell culture.

Specifications

Kit Components