Hypoxanthine-Thymidine (HT) Supplement (50x) for Hybridoma Cell Growth (TBS8073)

Description

Tribioscience’s HT Supplement Solution is a liquid mixture of hypoxanthine and thymidine (HT) for hybridoma cell selection, and antibody production.

It is used for the preparation of selection medium for hybridoma. The myeloma cell is deficient in the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT) or thymidine kinase (TK) and cannot survive in HT selection medium. This selection Solution has been verified for use in hybridoma development and monoclonal antibody production.

Main Components

This product is a chemically defined liquid medium. The main components as below:
Hypoxanthine (H)
Thymidine (T)

Applications

• Suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.