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Human VEGF Fast ELISA
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The TriboTM Fast Human VEGF ELISA is designed to quantitatively detect Human VEGF levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of human VEGF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human VEGF protein.
This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The minimum detectable dose (MOD) of human VEGF is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
This assay recognizes natural and recombinant human VEGF.
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human VEGF Microplate | TBS3264A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human VEGF. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human VEGF Standard | TBS3264B | 30 µl of Recombinant human VEGF protein (50ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3264C | 2.1 ml of Biotin-Human VEGF antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3264D | 300 µl of Streptavidin-HRP. | |
| Assay Diluent | TBS3264E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
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