| Description |
Recombinant Human Peptide Ready HLA-B*07:02&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Val309 (HLA-B*07:02) and Ile21-Met119 (B2M). |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
| Accession |
P01889(HLA-B*07:02)&P61769(B2M) |
| Target Symbol |
Peptide Ready HLA-B*07:02&B2M Monomer |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His-Avi |
| Expression Range |
Gly25-Val309(HLA-B*07:02) & Ile21-Met119(B2M) |
| Mol. Weight |
The protein has a predicted MW of 48.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human Peptide Ready HLA-B*07:02&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Peptide Ready HLA-B*07:02&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-B*07:02. Peptide ready MHC molecules comprising human HLA-B*07:02 alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
