The TribioTM Fast Human Lactoferrin ELISA is designed to quantitatively detect Human Lactoferrin levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 15.6 to 1000 pg/mL. The levels of human Lactoferrin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human Lactoferrin protein.
Typical Data
This standard curve (R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human Lactoferrin is typically 10 pg/ml.
The Intra-assay CV is 3.79%, the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human Lactoferrin.
