| Description |
Recombinant Human Integrin alpha X beta 2(ITGAX&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Pro1107(ITGAX) acidic tail & Gln23-Asn700(ITGB2) basic tail. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
P20702-1(ITGAX)&P05107-1(ITGB2) |
| Target Symbol |
Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer |
| Synonyms |
CD11C; SLEB6; CD18; ITGAX&ITGB2 ITGA; ITGB2 |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Phe20-Pro1107(ITGAX) acidic tail & Gln23-Asn700(ITGB2) basic tail |
| Mol. Weight |
The protein has a predicted MW of 125.50 kDa(ITGAX)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 130-135 kDa(ITGAX)&85-95 kDa(ITGB2) kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human ITGAX&ITGB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response. |
