| Description |
Recombinant Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
P06756-1(ITGAV)&P05556-1(ITGB1) |
| Target Symbol |
Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His-Avi |
| Expression Range |
Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail |
| Mol. Weight |
The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells. |
