| Description |
Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700 (ITGB2) basic tail. |
| Purity |
> 95% as determined by Tris-Bis PAGE |
| Accession |
P11215-1(ITGAM)&P05107-1(ITGB2) |
| Target Symbol |
Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Phe17-Asn1104 (ITGAM) acidic tail & Gln23-Asn700 (ITGB2) basic tail |
| Mol. Weight |
The protein has a predicted MW of 126.10 kDa (ITGAM) and 79.50 kDa (ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM) and 90-100 kDa (ITGB2) based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human ITGAM&ITGB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DCSIGN[2]. |
