The Fast Human IL-3 ELISA is a solid phase ELISA designed to measure human IL-3 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not needing 4-5 hours (Fig. 1). The detection range is from 15.6 to 1000 pg/mL. The levels of human IL-3 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-3 protein.
Typical Data
This standard curve (R2=0.9925) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human IL-3 is typically 3.0 pg/ml.
The Intra-assay CV is 4.9% the Inter-assay CV is 8.1%.
Specificity
This assay recognizes natural and recombinant human IL-3.
No cross-reactivity: human IL-1α; human IL-1β; Mouse IL-1β; Mouse IL-3; Mouse IL-4; Mouse IL-5.
