The Tribio® Human IFN-α Fast ELISA is a solid phase ELISA designed to measure human IFN-α levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 15.6 to 1000 pg/mL. The levels of human IFN-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN-α protein.
Typical Data
This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IFN-α is typically 8 pg/ml.
Specificity
This assay recognizes natural and recombinant human IFN-α.
Simple Procedures (Fig. 1)
