| Description |
Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein is expressed from HEK293 with His tag and Avi at the C-Terminus.It contains Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPKTLVL peptide. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
P13747(HLA-E*01:03)&P61769(B2M)&VMAPKTLVL |
| Target Symbol |
HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His-Avi |
| Expression Range |
Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPKTLVL peptide |
| Mol. Weight |
The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes. |
