| Description |
Recombinant Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)& HMTEVVRHC |
| Target Symbol |
HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer |
| Synonyms |
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53 |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His-Avi |
| Expression Range |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide |
| Mol. Weight |
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 44.7ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth. |
