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Human EGFR ELISA
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The TriboTM Fast Human EGFR ELISA is designed to quantitatively detect Human EGFR levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 3 to 200 pg/mL. The levels of human EGFR samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human EGFR protein.
This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The minimum detectable dose (MOD) of human EGFR is typically 2 pg/ml.
The Intra-assay CV is 3.79%, the Inter-assay CV is ˂10%.
This assay recognizes natural and recombinant human EGFR.
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human EGFR Microplate | TBS3250A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human EGFR. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human EGFR Standard | TBS3250B | 15 µl of Recombinant human EGFR protein (20ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3250C | 2.1 ml of Biotin-Human EGFR antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3250D | 300 µl of Streptavidin-HRP. | |
| Assay Diluent | TBS3250E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
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