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Human Complement AH50 / CAH 50 Fast ELISA
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The TribioTM Fast Human CAH50 ELISA is designed to quantitatively detect Human CAH50 levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The levels of human CAH50 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human CAH50 protein.
This standard curve (R2=0.9997) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The minimum detectable dose (MOD) of human CAH50 is typically 5 pg/ml.
The Intra-assay CV is 3.79%, the Inter-assay CV is ˂10%.
This assay recognizes natural and recombinant human CAH50.
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human CAH50 Microplate | TBS3267A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human CAH50. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human CAH50 Standard | TBS3267B | 30 µl of Recombinant human CAH50 protein (25 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3267C | 2.1 ml of Biotin-Human CAH50 antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3267D | 300 µl of Streptavidin-HRP. | |
| Assay Diluent | TBS3267E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
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