Description
TaqMan RT-PCR Hop Latent Viroid (HLVd) Detection Kit is a one-step real-time reverse transcription polymerase chain reaction (RT-PCR) assay intended for the detection of HLVd infection in plant samples. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy to use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting HLVd.
TaqMan RT-PCR Hop Latent Viroid Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer-probe, internal control, positive control, negative control, and buffer. The HLVd target gene is labeled with fluorescence Fam, and internal control is labeled with Hex.
APPLICATIONS
This kit is used for the detection of HLVd infection in plant samples.
KEY FEATURES
- One-step complete qRT-PCR in a single tube.
- Reduce contamination in the operating process.
- Accurate detection and quantification of HLVd target gene.
- 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.
STORAGE CONDITIONS
Store all components at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receipt. The kit is shipped on ice.
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