Hexokinase Activity Colorimetric Assay (TBS2107C)

Description

Hexokinase (EC 2.7.1.1, HK, ATP-dependent Hexokinase) plays an important role in glucose metabolism. Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis.

Tribioscience’s Hexokinase Activity Colorimetric Assay kit provides a flexible, accurate, sensitive, and time-saving approach for detecting HK activity in a wide variety of samples. In this assay, HK converts glucose into glucose-6-phosphate (G6P), then undergoes a series of reactions and induces the Colorimetric-Probe to generate a strong colored product detected at 460 nm.

Features

• Flexible: Suitable for 96-well or 384-well plate.
• Accuracy: Absorbance measurement is proportional to the HK concentration.
• Sensitive: Can detect as low as 7 mU/mL.
• Time-saving: Just incubate for 30 minutes and read out.

Applications

• Hexokinase activity in different samples like cells and tissues.

Standard Preparation

Standard Curve