Glycerol Colorimetric/Fluorometric Assay

Description

Glycerol is a central component for the synthesis of all lipids and acts as a backbone for triglycerides and phospholipids. It is released from triglycerides as the product of lipolysis. The measurements of circulating glycerol are useful parameters to evaluate lipolysis under various conditions in clinical studies.

Tribioscience’s Glycerol Colorimetric Assay kit is a robust, simple, and high throughput method based on coupled enzyme reactions. Glycerol is phosphorylated and oxidized to generate hydrogen peroxide, which is catalyzed and reacts with the probe measured by the colorimetric method at OD = 570 nm.

Assay Principle

Applications

This assay can detect as low as 1 µg/mL of glycerol in a variety of biological samples, including food, beverage, cell culture medium, cell lysate, tissues and serum, and plasma samples.

Storage Conditions

Store kit at -20°C. Shelf life of 12 months.