Glucose-6-Phosphate Colorimetric Assay (TBS2103)

Description

Glucose 6-Phosphate dehydrogenase (G6PDH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. In the presence of NADP+ or NAD+, it oxidizes glucose-6-phosphate (G6P) to 6-Phosphogluconolactone (6PG) and NADPH or NADH. It plays a vital role in numerous biosynthetic pathways and is important for preventing oxidative damage. In humans, deficiency is an autosomal hereditary disorder that can result in the depletion of NADPH in red blood cells and increases the risk of hemolytic anemia in situations of oxidative stress. On the other hand, inhibition of G6PDH has been linked to anti-cancer activity by decreasing RNA biosynthesis and increasing the buildup of reactive oxygen species in tumor cells.

Tribioscience’s G6PDH Activity Colorimetric Assay kit provides a flexible, accurate, sensitive, and time-saving approach for detecting G6PDH activity in a wide variety of samples.

In this assay, G6PDH converts glucose-6-phosphate to 6-phosphogluconate and NAD(P)H, which in turn induces the Colorimetric Probe to generate an intense colored product at 460 nm as shown in Fig.1.

Fig.1. Principle of the Assay

Features

• Flexible: Suitable for 96-well plate.
• Accuracy: Absorbance measurement is proportional to the G6PDH concentration.
• Sensitive: Can detect as low as 0.2 mU/mL.
• Time-saving: Just incubate and read out style.

Applications

• Measure G6PDH activity in a variety of samples like cells and tissues.

Fig.2. Typical standard curve of NADH