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Fast Human Thyroid Stimulating Hormone β / TSHβ ELISA, 1 step and 1hr
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The Fast Human TSHβ ELISA is a solid phase ELISA designed to measure human TSHβ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The detection range is from 31 to 2000 pg/mL. The levels of human TSHβ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TSHβ protein.
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The minimum detectable dose (MOD) of human TSHβ is typically 5 pg/ml.
The Intra-CV is < 10%, the Inter-CV is < 12%.
This assay recognizes natural and recombinant human TSHβ.
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human TSHβ Microplate | TBS3273A | 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human TSHβ. | The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C. |
| Human TSHβ Standard | TBS3273B | 30 μL of Recombinant human TSHβ protein (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3273C | 2.1 mL of human TSHβ antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3273D | 12 mL of Streptavidin-HRP | |
| Assay Diluent | TBS3273E | 12 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
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