Fast Glucose Oxidase Activity Assay (Colorimetric/Fluorometric)

Description

Glucose oxidase (GOD) catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct, and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery.
Tribioscience’s Fast Glucose Oxidase Activity Assay uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample. The fluorometric assay is more sensitive than the colorimetric assay.

Applications:

Direct Assay: Glucose oxidase activity in serum, plasma, urine, and other bio-samples.

Key Features:

Flexible: Suitable for colorimetric and fluorometric methods.
Accurate: Use 50μL samples. Detection ranges 0.1-50 μM in 96-well plate for colorimetric assay and 0.002-10 μM for fluorometric assay.
Simple and High-Throughput: One-step procedure. Load-Incubate-Read. The kit can be used for a robust method.
Time Saving: takes less than 30 minutes.

Kit Contents:

Storage and Handling:

Store kit at -20℃.
Shelf life of 6-months.
Protect from light.

Example Fluorometric Standard Curve:

Example Colorimetric Standard Curve: