Exprep Plasmid SV (Midi)

Description

Exprep Plasmid SV Kit Midi DNA Purification kit provides a rapid and convenient method for the small and medium scale preparations of plasmid DNA from bacterial cells and it is used to isolate and purify any plasmids from any E. coli strains. Exprep™ Plasmid SV eliminates the need for organic solvent extraction and alcohol precipitation, allowing rapid and convenient preparation of many samples simultaneously.
Exprep Plasmid SV kit Mini can yield up to 30 μg (mini) of highly purified plasmid DNA and it can be applied directly for PCR, cloning, automated sequencing, synthesis of labeled hybridization probes, and other enzymatic reactions without further manipulation. ExprepTM Plasmid SV Midi kit procedure requires the centrifuge which has a swinging-out bucket and ability of 4,000 ~ 5,000 xg.

Applications:

Plasmid DNA purification on a small scale.

Key Features:

• Spin or vacuum column format.
• Stable and consistent result.
• Instant use: No need for additional materials.
• Fast and simple procedure: complete in 25 minutes
• High purity : A260/280 = 1.8 ~ 2.0
• Compatible with endA+ strains.
• No use of organic solvents.
• Ready for use in enzymatic manipulation and automated sequencing.

Storage Conditions:

GeneAll® Exprep™ Plasmid kit is shipped at room temperature. All components are stable at room temperature until the date of expiration that is printed on the product label. After the addition of RNase A, buffer S1 is stable for 1 year when stored at 4℃. In cold ambient conditions, buffer S2 and S3 may exhibit salt precipitation and this will cause a reduction of DNA recover-yields. If so, heat the bottle with occasional swirling in 37 ℃ water bath until completely dissolved.