Description
Exgene™ Genomic DNA micro kit provides fast and easy methods for the micro scale purification of total (genomic and mitochondrial) DNA from various biological samples. Purified DNA can be used directly for PCR, quantitative PCR, genotyping such as STR analysis, and other downstream applications. Exgene™ Genomic DNA micro utilizes advanced silica-binding technology to purify total DNA sufficiently pure for many applications. Various samples are lysed in an optimized buffer containing detergents and lytic enzymes. Under the high salt condition, DNA in the lysate bind to the silica membrane, and impurities pass through the membrane into a collection tube. The membranes are washed with a series of alcohol-containing buffers to remove any traces of proteins, cellular debris, and salts. Finally, pure DNA is released into a clean collection tube with deionized water or low ionic strength buffer.
Kit Sizes
115-050: 50 samples
Applications
Microscale purification of total (genomic and mitochondrial) DNA from various biological samples.
Key Features
- Spin column format.
- Apply to trace of sample: use of microcolumn.
- Simple and safe procedure.
- Stable and consistent result.
- Instant use: No need for additional materials.
- No use of organic solvents.
- High yield and purity.
- Various protocols for forensic samples: stain, chewing gum, cigarette butts, toothbrush.
- Ready for use in general PCR, qPCR, genotyping such as STR analysis, and other downstream applications.
Storage Conditions
All components of GeneAll® Exgene™ Genomic DNA micro kit should be stored at room temperature (15~25℃). After reconstitution of proteinase K with storage buffer, it should be stored under 4°C for conservation of activity. It can be stored at 4℃ for 1 year without a significant decrease in activity. But for prolonged preservation of activity, storing under -20℃ is recommended. Under cool ambient conditions, a precipitate can be formed in buffer CL and/or BL. In such a case, heat the bottle above 37℃ to dissolve completely. GeneAll® Exgene™ Genomic DNA micro kit is guaranteed until the expiration date printed on the product label.
