Exgene™ Stool DNA Mini (DNA isolation)

Description

GeneAll Exgene™ Stool DNA mini kit provides a convenient method for the isolation of total DNA from stool samples. This kit utilizes a double binding procedure using the optimized buffer system and the advanced silica binding technology to purify nucleic acid suitable for many applications. Through this method, the contained impurities in the starting stool samples are removed so that high-quality DNA can be purified from the host and microbial cells. The stool samples can be applied up to 200 mg per prep and this procedure can be completed in 25 minutes. This procedure is started with homogenization and lysis steps. The lysate is applied to the EzPassTM filter and then the stool DNA is eluted by centrifugation, the first binding step. After the first elution, the eluate is mixed with DNA binding buffer and the stool DNA is bound on the silica membrane. Following the washing step, the bound DNA is eluted by elution buffer, the second elution. Purified DNA can be directly applicable in conventional PCR, restriction analysis, electrophoresis, and any other downstream applications.

Kit Sizes

115-150: 50 samples

Key Features

• Stable and consistent DNA extraction from stool samples.
• Sample size: Up to 200 mg.
• Spin column format.
• Purification of high-quality DNA by the use of EzPassTM filter column.
• Stable and consistent yield.
• No organic extraction or alcohol precipitation.
• Ready for use in PCR, restriction analysis, electrophoresis, and any other downstream applications.

Storage Conditions

GeneAll® Exgene™ Stool DNA mini kit should be stored at room temperature (15~25℃). In cold ambient conditions, buffer FL and PB may exhibit salt precipitation and this will cause a reduction in DNA recover yields. If so, heat the bottle with occasional swirling in a 37℃ water bath until completely dissolved. All components are stable for 1 year.