Exgene Plant SV (DNA isolation, Midi)

Description

Exgene™ Plant SV kit provides a simple and easy method for the small, medium, and large-scale purification of total DNA from various plant tissues. With EzSep™ filter and column type G, the procedure can be done in just 40 minutes (mini), yielding a pure genomic DNA suitable for various downstream applications without further manipulation. Up to 100 mg and 400 mg of plant tissue can be processed with Exgene™ Plant SV mini, Midi, and MAXI, respectively. Exgene™ Plant SV procedure eliminates the need for organic solvent extraction and alcohol precipitation, allowing safe and fast preparation of many samples simultaneously. Purified total DNA can be directly applicable in conventional PCR, real-time PCR, Southern blotting, SNP genotyping, RFLP, AFLP, and RAPD.

Kit Sizes

117-226: 26 samples

Key Features

• Spin or vacuum column format.
• Stable and consistent DNA extraction from plant cells, tissues, and fungi.
• Instant use: No need for additional materials.
• Perfect removal of second metabolites such as polyphenols and polysaccharides.
• Simple procedure by the use of EzSep™ filter column.
• No use of organic solvents.
• Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD, and other enzymatic reactions.

Applications

Purification of total DNA from various plant tissues on a medium scale.

Storage Conditions

All components of GeneAll® Exgene™ Plant SV kit should be stored at room temperature (15~25℃). RNase A is delivered under ambient conditions and can be stored at room temperature for 6 months without a significant decrease in activity. But for prolonged conservation of activity, storing at -20 ~ 8℃ is recommended. During delivery or storage under cold ambient conditions, a precipitate may be formed in buffer PL. Heat the bottle to dissolve completely before use. Using precipitated buffers will lead to poor DNA recovery. GeneAll® Exgene™ Plant SV kit series are guaranteed for 1 year.