DBIC, DNMT3B S-nitrosylation inhibitor (TBI5161)

Description

Product Details

Formal Name: (E)-N’-(3,4-Dihydroxybenzylidene)-1H-benzo[d]imidazole-5-carbohydrazide

Molecular Formula: C₁₅H₁₂N₄O₃

Formula Weight: 296.30

CAS Number: 487026-67-5

Purity: >98%

Formulation: powder

Solubility: Soluble in DMSO (30 mg/ml)

Storage: -20°C

Stability: ≥ 2 years.

Applications

DNMT3B S-nitrosylation inhibitor

Functions

Specifically inhibits S-nitrosylation of DNA methyltransferase 3B (DNMT3B) at low concentrations, IC50≤100 nM, without affecting its enzymatic activity. Treatment with DBIC prevents nitric oxide-induced conversion of human colonic adenoma to adenocarcinoma in vitro. Treatment with DBIC strongly attenuates tumor development in a mouse model of carcinogenesis triggered by inflammation-induced NO production. DNMT3B-mediated DNA methylation is regulated by S-nitrosylaton and DBIC represents a new useful tool for studying this system. A negative control compound, DBIC-neg2 (Cat # MC-5162) is also available.

Application Procedures

First dissolved in DMSO (30 mg/ml), then diluted to aqueous buffer. S Solutions in DMSO may be stored at -20°C for up to 1 month.