Cytochrome C Reductase Activity Assay

Description

This kit is designed to measure the NADPH cytochrome C reductase activity in biological samples including cell and tissue extracts, and in purified microsomes (endoplasmic reticulum, ER). Eukaryotic NADPH-Cytochrome C reductase (NADPH cytochrome P450 reductase, EC 1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which is the cytochrome P450 family of enzymes, responsible for xenobiotic metabolism.

NADPH-cytochrome C reductase is widely used as an endoplasmic reticulum marker and as a biomarker of ecological pollution and dietary lipid uptake. This kit is based on a colorimetric assay that measures the reduction of cytochrome C by NADPH-Cytochrome C reductase in the presence of NADPH. The reduction of cytochrome C results in the formation of distinct bands in the absorption spectrum and the increase in absorbance at 550 nm is measured with time.

Applications

• Measuring NADPH-cytochrome c reductase levels in biological samples after exposure to drugs or other xenobiotics.
• Detection of ER during isolation and subcellular fractionation by density gradient separation.

Key Features

• Flexible: The kit can be used for 96-well plates or 384-well plates.
• Accurate: Accurate detection of cytochrome C reductase activity in small size as 50 µL samples.
• Simple: Just load-Read. The kit can be used for a robust method.
• Time-saving: Less than 30 minutes.

Kit Contents

Storage Conditions

Store kit at -20°C. Shelf life of 2 years. Protect from light.
Ship with blue ice.

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