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Product Description
Description
This assay kit is a competition enzyme-linked immunoassay designed to measure cAMP levels in cells or tissues. An anti-mouse IgG was pre-coated onto a microplate. The cAMP in the test sample competes with cAMP-HRP for binding to an anti-cAMP mouse monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cAMP levels. Measurement of absorbance using the cAMP Standard allows calculating the absolute amount of cAMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cAMP detection.
Typical Data
This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of cAMP is typically 1 nM.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes cAMP. No cross-reactivity with others.
Standard Preparation
Standard Curve (Fig.2)
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Precoated Microplate
TBS2073A
96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
cAMP Standard
TBS2073B
50 µL of cAMP (1 mM).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS2073C
110 µL of cAMP-HRP (25x).
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS2073D
1.1 mL of anti-cAMP antibody (5x).
Assay Diluent
TBS2073E
20 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
This assay kit is a competition enzyme-linked immunoassay designed to measure cAMP levels in cells or tissues. An anti-mouse IgG was pre-coated onto a microplate. The cAMP in the test sample competes with cAMP-HRP for binding to an anti-cAMP mouse monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cAMP levels. Measurement of absorbance using the cAMP Standard allows calculating the absolute amount of cAMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cAMP detection.
Typical Data
This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of cAMP is typically 1 nM.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes cAMP. No cross-reactivity with others.
Standard Preparation
Standard Curve (Fig.2)
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Precoated Microplate
TBS2073A
96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
cAMP Standard
TBS2073B
50 µL of cAMP (1 mM).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS2073C
110 µL of cAMP-HRP (25x).
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS2073D
1.1 mL of anti-cAMP antibody (5x).
Assay Diluent
TBS2073E
20 mL of a buffered protein base with preservatives.
Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
