Caspase-1 Fluorometric Assay Kit (TBS2045)

Description

Caspase-1 (IL-1β Converting Enzyme, ICE) is the prototypical member of the ICE family of cysteine proteases, also known as caspases (cysteinyl aspartate-specific proteases). Caspase-1 was first identified as a novel protease that generates the proinflammatory cytokine, interleukin-1β by cleaving pro-interleukin-1β. It plays an important role in inflammatory diseases and cancer.

Tribo^TM Caspase-1 Activity Fluorometric Assay Kit provides a simple and convenient method for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on the detection of the free AFC fluorescence after cleavage from the labeled substrate YVAD-AFC. The free AFC can be quantified using a fluorometer or a fluorescence microtiter plate reader at Ex400/Em505nm. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows the determination of the fold increase in caspase-1 activity.

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