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Product Description
Description
Tribioscience’s Botulinum Neurotoxin Type A (BoNT-A) ELISA is designed to quantitatively detect Botulinum Neurotoxin Type A (BoNT-A) levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 46.9 to 3000 pg/mL. The levels of Botulinum Neurotoxin Type A (BoNT-A) samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural Botulinum Neurotoxin Type A (BoNT-A) protein.
Typical Data
This standard curve (R2=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of BoNT-A is typically 46 pg/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant BoNT-A.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Botulinum Neurotoxin Type A Microplate
TBS31007A
96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for BoNT-A.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Botulinum Neurotoxin Type A Standard
TBS31007B
50 µl of Recombinant Botulinum Neurotoxin Type A (30 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS31007C
2.1 mL of biotin-Botulinum Neurotoxin Type A antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS31007D
200 µL of streptavidin HRP.
Assay Diluent
TBS31007E
25 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Tribioscience’s Botulinum Neurotoxin Type A (BoNT-A) ELISA is designed to quantitatively detect Botulinum Neurotoxin Type A (BoNT-A) levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 46.9 to 3000 pg/mL. The levels of Botulinum Neurotoxin Type A (BoNT-A) samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural Botulinum Neurotoxin Type A (BoNT-A) protein.
Typical Data
This standard curve (R2=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of BoNT-A is typically 46 pg/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant BoNT-A.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part
Part#
Description
Storage of Opened/Reconstituted
Botulinum Neurotoxin Type A Microplate
TBS31007A
96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for BoNT-A.
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Botulinum Neurotoxin Type A Standard
TBS31007B
50 µl of Recombinant Botulinum Neurotoxin Type A (30 ng/mL).
Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A
TBS31007C
2.1 mL of biotin-Botulinum Neurotoxin Type A antibody.
May be stored for up to 3 months at 2-8 °C.
Detection B
TBS31007D
200 µL of streptavidin HRP.
Assay Diluent
TBS31007E
25 mL of a buffered protein base with preservatives.
10x Wash Buffer
TBS3000W
12 mL of concentrated solution (10x).
TMB Substrate
TBS3000T
12 mL of ultra-sensitive TMB substrate.
Stop Solution
TBS3000S
6 mL of 2 N sulfuric acid.
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
