Description
The Bacillus Species qPCR Kit has been designed specifically to identify the Bacillus species in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescent labels. The detection of the target DNA confirms ingredient authenticity and prevents food fraud, ethical issues, or health concerns.
Tribioscience’s Bacillus Species qPCR Kit includes Bacillus positive and negative controls, PCR internal controls labeled with Hex, a qPCR super mix, and the primer probe mix in which the probe has been labeled with FAM for the target gene. These aid in a straightforward interpretation of the results.
Applications
Detect Bacillus-derived DNA in the plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed.
Key Features
- High sensitivity and specificity for all Bacillus cereus species.
- High efficiency: the optimal systemic conditions for PCR amplification.
- Streamlined protocol: just add DNA template and water.
- No cross reactivity with other species.
Kit Contents
Name |
100x rxn |
200x rxn |
qPCR Super Mix (B1) |
0.8mL |
1.6mL |
Primer-Probe Mix (B2) |
0.6mL |
1.2mL |
Positive Control DNA (B+) |
60μL |
100μL |
Negative Control DNA (B–) |
60μL |
100μL |
The bacillus target probe has been labeled with FAM while the PCR internal control has been labeled with Hex
Storage Conditions
The kit is shipped on ice and stored at -20℃ for long term storage.
Shelf life of 12 months after receiving.