Allspin (RNA & DNA isolation)

Description

Allspin™ total DNA/RNA purification kit provides a convenient method for the isolation of total DNA and total RNA simultaneously from a single sample of tissue or cultured cells. DNA and RNA are purified separately from the same sample by an individual but successive procedures using column B and column W respectively. The whole procedure can be performed in just 30 minutes and the length of obtained DNA is up to 50 kb (average is 30 kb) and that of RNA is longer than 200 nucleotides.

Applications

Simultaneous isolation of total DNA and total RNA from a single sample of tissue or cultured cells.

Key Features

• Glassfiber membrane technology.
• Sample size: Up to 20mg tissue or up to 1x 10⁷ cultured cells.
• Typical yield of RNA: Up to 20 μg/1x 10⁶ cultured cells Up to 60 μg/10mg liver tissue.
• Typical yield of DNA: Up to 10 μg/1x 10⁶ cultured cells Up to 25 μg/10mg liver tissue.
• High purity.
• Preparation time: ~30 minutes.
• Stable and consistent yield.
• No phenol / chloroform extraction.
• No ethanol precipitation.
• Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assays, and other analytical procedures.

Storage Conditions

Allspin™ total DNA/RNA purification Kit should be stored at room temperature. All components are stable for 1 year.