Alkaline Phosphatase Activity Fluorometric Assay (TBS2078)

Description

Tribioscience’s Alkaline Phosphatase Activity Fluorometric Assay Kit provides a highly sensitive and convenient way for direct detection of ALP activity in different biological samples. In the assay, ALP removes the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (4-MUP) substrate producing an intense fluorescent signal (Ex/Em = 360 nm/450 nm). The kit is an ultra-sensitive, HTS-ready assay that is more sensitive than colorimetric methods. The assay is suited for both research and drug discovery.

Applications

Direct Assays: Acid phosphatase in serum, plasma, urine, and other bio-samples.

Key Features

• Flexible: Suitable for colorimetric assay.
• Accurate: Use 50 µL samples. Detection ranges 0.3-80 U/L in 96-well plate for fluorometric assay.
• Simple and high-throughput: Just load-incubate-Read. The kit can be used as a robust method.
• Time-saving: 20-30 minutes.

Standard Curve Preparations (Fig. 1)

Typical Standard Curve (Fig. 2)

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