Acid Phosphatase Activity Colorimetric Assay (TBS2075)

Description

Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic index for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.

Tribioscience’s Acid Phosphatase Colorimetric Assay Kit provides a high-sensitive, simple, and direct assay approach to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect as low as 20 µU acid phosphatase activity in samples.

Applications

Direct Assays: Acid phosphatase in serum, plasma, urine, and other bio-samples.

Key Features

• Flexible: Suitable for colorimetric assay.
• Accurate: Use 50 µL samples. Detection ranges from 0.4-200 µU in a 96-well plate for colorimetric assay.
• Simple and high-throughput: Just load-incubate-Read. The kit can be used for a robust method.
• Time-saving: less than 30 minutes.

Standard Curve Preparations

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